Dr. Devaraja Mudeppa

Devaraja Mudeppa is a Senior Research Scientist. He works on addressing malarial recombinant protein expression challenges using in-house developed wheat embryo cell-free protein expression platform. These expressed recombinant proteins are utilized in the lab for inhibitor screening, developing antigen arrays and for structural biology studies. He is actively involved in NIH sponsored MESA-ICEMR and Topoisomerase projects and helping graduate students to meet their project goals. He completed his Ph.D. studies at Central Food Technological Research Institute, India and post-doctoral studies at Ehime University, Japan. Dev has two children.

Email: devmg@uw.edu

Publications

Kumar, S, Mudeppa, DG, Sharma, A, Mascarenhas, A, Dash, R, Pereira, L et al.. Distinct genomic architecture of Plasmodium falciparum populations from South Asia. Mol. Biochem. Parasitol. ;210 (1-2):1-4. doi: 10.1016/j.molbiopara.2016.07.005. PubMed PMID:27457272PubMed Central PMC5249597.

Mudeppa, DG, Kumar, S, Kokkonda, S, White, J, Rathod, PK. Topoisomerase II from Human Malaria Parasites: EXPRESSION, PURIFICATION, AND SELECTIVE INHIBITION. J. Biol. Chem. 2015;290 (33):20313-24. doi: 10.1074/jbc.M115.639039. PubMed PMID:26055707 PubMed Central PMC4536438.

Kumar, S, Krishnamoorthy, K, Mudeppa, DG, Rathod, PK. Structure of Plasmodium falciparum orotate phosphoribosyltransferase with autologous inhibitory protein-protein interactions. Acta Crystallogr F Struct Biol Commun. 2015;71 (Pt 5):600-8. doi: 10.1107/S2053230X1500549X. PubMed PMID:25945715 PubMed Central PMC4427171.

Mudeppa, DG, Rathod, PK. Expression of functional Plasmodium falciparum enzymes using a wheat germ cell-free system. Eukaryotic Cell. 2013;12 (12):1653-63. doi: 10.1128/EC.00222-13. PubMed PMID:24123271 PubMed Central PMC3889570.

Mailu, BM, Ramasamay, G, Mudeppa, DG, Li, L, Lindner, SE, Peterson, MJ et al.. A nondiscriminating glutamyl-tRNA synthetase in the plasmodium apicoplast: the first enzyme in an indirect aminoacylation pathway. J. Biol. Chem. 2013;288 (45):32539-52. doi: 10.1074/jbc.M113.507467. PubMed PMID:24072705 PubMed Central PMC3820887.

Sun, XE, Sharling, L, Muthalagi, M, Mudeppa, DG, Pankiewicz, KW, Felczak, K et al.. Prodrug activation by Cryptosporidium thymidine kinase. J. Biol. Chem. 2010;285 (21):15916-22. doi: 10.1074/jbc.M110.101543. PubMed PMID:20231284 PubMed Central PMC2871459.

Larson, ET, Mudeppa, DG, Gillespie, JR, Mueller, N, Napuli, AJ, Arif, JA et al.. The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase. J. Mol. Biol. 2010;396 (5):1244-59. doi: 10.1016/j.jmb.2010.01.013. PubMed PMID:20070944 PubMed Central PMC2844865.

Pang, CK, Hunter, JH, Gujjar, R, Podutoori, R, Bowman, J, Mudeppa, DG et al.. Catalytic and ligand-binding characteristics of Plasmodium falciparum serine hydroxymethyltransferase. Mol. Biochem. Parasitol. 2009;168 (1):74-83. doi: 10.1016/j.molbiopara.2009.06.011. PubMed PMID:19591883 PubMed Central PMC2741015.

Mudeppa, DG, Pang, CK, Tsuboi, T, Endo, Y, Buckner, FS, Varani, G et al.. Cell-free production of functional Plasmodium falciparum dihydrofolate reductase-thymidylate synthase. Mol. Biochem. Parasitol. 2007;151 (2):216-9. doi: 10.1016/j.molbiopara.2006.10.016. PubMed PMID:17157934 .